As a soloist, he has worked with conductors such as William Christie, Jordi Savall, Marcel Peres, Philippe Herreweghe, Marc Minkowski, and others.

In 1985, Lesne founded his own group: Il Seminario Musicale. Since then, he has devoted the bulk of his time to this group, both as a singer and as a music director. Il Seminario Musicale specializes in Italian and French baroque repertoir. Many of their recordings have won prestigious prizes such as Diapason d'Or, ffff Telerama, Choc du Monde de la Musique and Timbre de Platine.

In addition to working with his own group, Lesne occasionally collaborates with his sister Brigitte, who is herself a fine alto.